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Leveraging Baird aromaticity for advancement of bioimaging applications. Martin MI, Pati AK, Abeywickrama CS, Bar S, Kilic Z, Altman RB, Blanchard SC. J. PHYS. ORGANIC CHEMISTRY (2022) doi: 10.002/poc.4449

Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes. Juette MF, Carelli JD, Rundlet EJ, Brown A, Shao S, Ferguson A, Wasserman MR, Holm M, Taunton J, Blanchard SC.  ELIFE (2022) Vol.11:e81608. doi: 10.7554/eLife.81608. PMID: 36264623; PMCID: PMC9584604.


Synthesis and single-molecule imaging reveal stereospecific enhancement of binding kinetics by the antitumour eEF1A antagonist SR-A3. Wang HY, Yang H, Holm M, Tom H, Oltion K, Al-Khdhairawi AAQ, Weber JF, Blanchard SC, Ruggero D, Taunton J.  NATURE CHEMISTRY (2022) Sep 19. doi: 10.1038/s41557-022-01039-3. Epub ahead of print. PMID: 36123449.

Hyper-swivel head domain motions are required for complete mRNA- tRNA translocation and ribosome resetting. Nishima W, Girodat D, Holm M, Rundlet EJ, Alejo JL, Fischer K, Blanchard SC,
Sanbonmatsu KY.  NUCLEIC ACIDS RESEARCH 2022 Vol. 50(14):8302-8320. doi: 10.1093/nar/gkac597. PMID: 35808938; PMCID: PMC9371933.

The cyclic octapeptide antibiotic argyrin B inhibits translation by trapping EF-G on the ribosome during translocation. Wieland M, Holm M, Rundlet EJ, Morici M, Koller TO, Maviza TP, Pogorevc D, Osterman IA, Müller R, Blanchard SC, Wilson DN.  PNAS (2022) May 10;119(19):e2114214119. doi: 10.1073/pnas.2114214119. Epub 2022 May 2. PMID: 35500116; PMCID:

GPCR-mediated β-arrestin activation deconvoluted with single-molecule precision. Asher WB, Terry DS, Gregorio GGA, Kahsai AW, Borgia A, Xie B, Modak A, Zhu Y, Jang W, Govindaraju A, Huang LY, Inoue A, Lambert NA, Gurevich VV, Shi L, Lefkowitz RJ, Blanchard SC, Javitch JA.  CELL. 2022 Vol.185(10):1661-1675.e16. doi: 10.1016/j.cell.2022.03.042. Epub 2022 Apr 27. PMID: 35483373; PMCID: PMC9191627.


Linking function to global and local dynamics in an elevator-type transporter. Ciftci D, Martens C, Ghani VG, Blanchard SC, Politis A, Huysmans GHM, Boudker O. PNAS (2021) Dec 7;118(49):e2025520118. doi: 10.1073/pnas.2025520118. PMID: 34873050; PMCID: PMC8670510.

The chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma. |Pan M, Wright WC, Chapple RH, Zubair A, Sandhu M, Batchelder JE, Huddle BC, Low J, Blankenship KB, Wang Y, Gordon B, Archer P, Brady SW, Natarajan S, Posgai MJ, Schuetz J, Miller D, Kalathur R, Chen S, Connelly JP, Babu MM, Dyer MA, Pruett-Miller SM, Freeman BB 3rd, Chen T, Godley LA, Blanchard SC, Stewart E, Easton J, Geeleher P. NATURE COMMUNICATIONS (2021) Vol.12(1):6468. doi: 10.1038/s41467-021-26640-x. PMID: 34753908; PMCID:PMC8578635.


Translation Elongation in Bacteria. Blanchard SC, Holm M. & Rundlet EJ. Encyclopedia of Biological Chemistry III (Third Edition) (2021) Elsevier, Editor: Joseph Jez: Pages 555-563.

Structural basis of early translocation events on the ribosome. Rundlet EJ, Holm M, Schacherl M, Natchiar SK, Altman RB, Spahn CMT, Myasnikov AG, Blanchard SC. NATURE (2021) Vol.595(7869):741-745. PMID: 34234344; PMCID: PMC8318882.

Single-molecule FRET imaging of GPCR dimers in living cells. Asher WB, Geggier P, Holsey MD, Gilmore GT, Pati AK, Meszaros J, Terry DS, Mathiasen S, Kaliszewski MJ, McCauley MD, Govindaraju A, Zhou Z, Harikumar KG,
Jaqaman K, Miller LJ, Smith AW, Blanchard SC, Javitch JA. NATURE METHODS (2021) Vol.18(4):397-405. PMID: 33686301; PMCID: PMC8232828


FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices. Lerner E, Barth A, Hendrix J, Ambrose B, Birkedal V, Blanchard SC, Börner R, Sung Chung H, Cordes T, Craggs TD, Deniz AA, Diao J, Fei J, Gonzalez RL, Gopich IV, Ha T, Hanke CA, Haran G, Hatzakis NS, Hohng S, Hong SC, Hugel T, Ingargiola A, Joo C, Kapanidis AN, Kim HD, Laurence T, Lee NK, Lee TH, Lemke EA, Margeat E, Michaelis J, Michalet X, Myong S, Nettels D, Peulen TO, Ploetz E, Razvag Y, Robb C, Schuler B, Soleimaninejad H, Tang C, Vafabakhsh R, Lamb DC, Seidel CA, Weiss S. ELIFE (2021) Vol. 10:e60416. PMID: 33779550; PMCID: PMC8007216.


FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution. Ciftci D, Huysmans GHM, Wang X, He C, Terry D, Zhou Z, Fitzgerald G, Blanchard SC, Boudker O. BIOPROTOCOL (2021) Vol.5;11(7): PMID: 33889664; PMCID: PMC8054209.


Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles. Cell HostMicrobe. Lu M, Uchil PD, Li W, Zheng D, Terry DS, Gorman J, Shi W, Zhang B, Zhou T, Ding S, Gasser R, Prévost J, Beaudoin-Bussières G, Anand SP, Laumaea A, Grover JR, Liu L, Ho DD, Mascola JR, Finzi A, Kwong PD, Blanchard SC, Mothes W. CELL HOST & MICROBE (2020) Vol.28(6):880-891.e8. PMID: 33242391; PMCID: PMC7664471.

Quantitative comparison between sub-millisecond time resolution single-molecule FRETmeasurements and 10-second molecular simulations of a biosensor protein. Girodat D, Pati AK, Terry DS, Blanchard SC, Sanbonmatsu KY. PLOS COMPUTATIONAL BIOLOGY (2020) Vol.16(11); PMID: 33151943; PMCID: PMC7643941.

The high-energy transition state of the glutamate transporter homologue GltPh. Huysmans GHM, Ciftci D, Wang X, Blanchard SC, Boudker O. EMBO J. (2021) Vol.40(1) PMID: 33185289; PMCID: PMC7780239.

Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores.  Pati AK, El Bakouri O, Jockusch S, Zhou Z, Altman RB, Fitzgerald GA, Asher WB, Terry DS, Borgia A, Holsey MD, Batchelder JE,  Abeywickrama C, Huddle BC, Rufa D, Javitch JA, Ottosson H, Blanchard SC PNAS (2020) PMID: 32913060;PMCID: PMC7533661.

Sub-nanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles. Li Z, Li W, Lu M, Bess J Jr, Chao CW, Gorman J, Terry DS, Zhang B, Zhou T, Blanchard SC, Kwong PD, Lifson JD, Mothes W, Liu J. NATURE STRUCTURAL & MOLECULAR BIOLOGY (2020) Jun 29. doi: 10.1038/s41594-020-0452-2. Epub ahead of print. PMID: 32601441.

Single-molecule transport kinetics of a glutamate transporter homolog shows static disorder. Ciftci D, Huysmans GHM, Wang X, He C, Terry D, Zhou Z, Fitzgerald G, Blanchard SC, Boudker O. SCIENCE ADVANCES (2020) Vol.6(22):eaaz1949. PMID: 32523985; PMCID: PMC7259943.

Elongation Factor Tu Switch I Element is a Gate for Aminoacyl-tRNA Selection. Girodat D, Blanchard SC, Wieden HJ, Sanbonmatsu KY. JOURNAL OF MOLECULAR BIOLOGY (2020) Vol.432(9):3064-3077. doi: 10.1016/j.jmb.2020.01.038. Epub 2020 Feb 13. PMID:32061931.

Elongation factor-Tu can repetitively engage aminoacyl-tRNA within the ribosome during the proofreading stage of tRNA selection. Morse JC, Girodat D, Burnett BJ, Holm M, Altman RB, Sanbonmatsu KY, Wieden HJ, Blanchard SC. PNAS (2020) Vol.117(7):3610-3620. PMID: 32024753; PMCID: PMC7035488.

Long-Acting BMS-378806 Analogues Stabilize the State-1 Conformation of the Human Immunodeficiency Virus Type 1 Envelope Glycoproteins. Zou S, Zhang S, Gaffney A, Ding H, Lu M, Grover JR, Farrell M, Nguyen HT, Zhao C, Anang S, Zhao M, Mohammadi M, Blanchard SC, Abrams C, Madani N, Mothes W, Kappes JC, Smith AB 3rd, Sodroski J. JOURNAL OF VIROLOGY. (2020) Vol.94(10):e00148-20. PMID: 32161177; PMCID: PMC7199401.

Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements. Henderson R, Lu M, Zhou Y, Mu Z, Parks R, Han Q, Hsu AL, Carter E, Blanchard SC, Edwards RJ, Wiehe K, Saunders KO, Borgnia MJ, Bartesaghi A, Mothes W, Haynes BF, Acharya P, Munir Alam S. NATURE COMMUNICATIONS (2020) Vol.24;11(1):520. PMID: 31980614; PMCID: PMC6981184.

X-ray structure of LeuT in an inward-facing occluded conformation reveals mechanism of substrate release. Gotfryd K, Boesen T, Mortensen JS, Khelashvili G, Quick M, Terry DS, Missel JW, LeVine MV, Gourdon P, Blanchard SC, Javitch JA, Weinstein H, Loland CJ, Nissen P, Gether U. NATURE COMMUNICATIONS (2020) Vol.11(1):1005. PMID: 32081981; PMCID: PMC7035281.

Shedding-resistant HIV-1 envelope glycoproteins adopt downstream conformations that remain responsive to conformation-preferring ligands. Lu M, Ma X, Reichard N, Terry DS, Arthos J, Smith AB 3rd, Sodroski JG,
Blanchard SC, Mothes W. JOURNAL OF VIROLOGY (2020) Jun 10:JVI.00597-20. PMID: 32522853. 


Quantifying secondary transport at single-molecule resolution. Fitzgerald GA, Terry DS, Warren AL, Quick M, Javitch JA, Blanchard SC. NATURE (2019) Nov;575(7783):528-534. doi: 10.1038/s41586-019-1747-5. Epub 2019 Nov 13. PMID: 31723269.

Cargo adaptors regulate stepping and force generation of mammalian dynein-dynactin. Elshenawy MM, Canty JT, Oster L, Ferro LS, Zhou Z, Blanchard SC, Yildiz A. NATURE CHEMICAL BIOLOGY 2019 Nov;15(11):1093-1101. doi: 10.1038/s41589-019-0352-0. Epub 2019 Sep 9. PMID: 31501589; PMCID: PMC6810841.

A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS. McMahon M, Contreras A, Holm M, Uechi T, Forester CM, Pang X, Jackson C, Calvert ME, Chen B, Quigley DA, Luk JM, Kelley RK, Gordan JD, Gill RM, Blanchard SC, Ruggero D. ELIFE (2019) Vol. 8. pii: e48847. doi: 10.7554/eLife.48847. PMID: 31478838; PMCID: PMC6776443.


The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor. LeVine MV, Terry DS, Khelashvili G, Siegel ZS, Quick M, Javitch JA, Blanchard SC, Weinstein H. PNAS (2019) Aug 6;116(32):15947-15956. doi: 10.1073/pnas.1903020116. Epub 2019 Jul 19. PMID: 31324743; PMCID: PMC6689989.

Conformational dynamics between transmembrane domains and allosteric modulation of a metabotropic glutamate receptor. Gutzeit VA, Thibado J, Stor DS, Zhou Z, Blanchard SC, Andersen OS, Levitz J. ELIFE (2019) Jun 7;8. pii: e45116. doi: 10.7554/eLife.45116. PMID: 31172948; PMCID: PMC6588349.

Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease. Prakash V, Carson BB, Feenstra JM, Dass RA, Sekyrova P, Hoshino A, Petersen J, Guo Y, Parks MM, Kurylo CM, Batchelder JE, Haller K, Hashimoto A, Rundqivst H, Condeelis JS, Allis CD, Drygin D, Nieto MA, Andäng M, Percipalle P, Bergh J, Adameyko I, Farrants AÖ, Hartman J, Lyden D, Pietras K, Blanchard SC, Vincent CT. NATURE COMMUNICATIONS (2019) Vol.10(1):2110. PMID:31068593.


ADAM10 controls the differentiation of the coronary arterial endothelium. Farber G., Parks MM., Lustgarten Guahmich N., Zhang Y., Monette S., Blanchard SC., Di Lorenzo A., Blobel CP. ANGIOGENESIS (2019) Vol.22(2):237-250. PMID.30446855.


Implications of sequence variation on the evolution of rRNA. Parks MM, Kurylo CM, Batchelder JE, Theresa Vincent C, Blanchard SC. CHROMOSOME RESEARCH. 2019 Vol.(1-2):89-93. Review. PubMed PMID: 30719681.

Understanding the role of intermolecular interactions between lissoclimides and the eukaryotic ribosome. Pellegrino S, Meyer M, Könst ZA, Holm M, Voora VK, Kashinskaya D, Zanette C, Mobley DL, Yusupova G, Vanderwal CD, Blanchard SC, Yusupov M. NUCLEIC ACIDS RESEARCH (2019) Feb 13. doi: 10.1093/nar/gkz053. [Epub ahead of print] PubMed PMID: 30759226.

Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET. Lu M, Ma X, Castillo-Menedez LR, Gorman J. Alsahafi N, Ermel U, Terry DS, Chambers M, Peng D, Zhang B, Zhou T, Reichard N, Wang K, Grover JR, Carman BP, Gardner MR, Nikic-Spiegel I, Finzi A, Kwong PD, Blanchard SC, Sodroski JG, Mothes W. NATURE (2019) Col.568(7752):415-419. PMID:30971821.


tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP hydrolysis. Fils J., Holm M, Rundlet EJ., Loerke J., Hilal T., Dabrowski M., Burger J., Mielke T., Blanchard SC., Spahn CMT., Budkevich TV. CELL REPORTS 2018 Vol.25(10):2676-2688.e7. PMID:30517857.


ERASE: A novel surface reconditioning strategy for single-molecule experiments. Broadwater DWB Jr., Altman RB., Blanchard SC, Kim HD. NUCLEIC ACIDS RESEARCH 2018 Nov. 20 PMID.30462308.

Kurylo CM, Parks, MM, Juette, MF, Zinshteyn, B, Altman, RB, Thibado, JK, Vincent, CT, Blanchard SC. Endogenous rRNA sequence variation can regulate stress response gene expression and phenotype. CELL REPORTS. 2018 Oct 2;25(1):235-248. PubMed PMID: 30282032.

Riederer EA, Focke PJ, Georgieva ER, Akyuz N, Matulef K, Borbat PP, Freed JH, Blanchard SC, Boudker O, Valiyaveetil FI. A facile approach for the in vitro assembly of multimeric membrane transport proteins. ELIFE. 2018 Jun 11;7. pii: e36478. PubMed PMID: 29889023.

Blanchard SC. A Much-Needed Boost for the Dwindling Antibiotic Pipeline. MOLECULAR CELL. 2018 Apr 5;70(1):3-5. PubMed PMID: 29625037.

Ma X, Lu M, Gorman J, Terry DS, Hong X, Zhou Z, Zhao H, Altman RB, Arthos J, Blanchard SC, Kwong PD, Munro JB, Mothes W. HIV-1 Env trimer opens through an asymmetric intermediate in which individual protomers adopt distinct conformations. ELIFE. 2018 Mar 21;7. pii: e34271. PubMed PMID: 29561264; PubMed Central PMCID: PMC5896952.

Parks MM, Kurylo CM, Dass RA, Bojmar L, Lyden D, Vincent CT, Blanchard SC. Variant ribosomal RNA alleles are conserved and exhibit tissue-specific expression. SCIENCE ADVANCES (2018) Vol.4(2). eaao0665.

Terry DS, Kolster RA, Quick M. LeVine MV, Khelashvilli G, Zhou Z, Weinstein H, Javitch JA, Blanchard SC. A partially-open inward-facing intermediate conformation of LeuT is associated with Na+-release and substrate transport. NATURE COMMUNICATIONS (2018) Vol.9(1).230. PMID: 29335402.




Prokhorova I, Altman RB, Djumagulov M, Shrestha JP, Urzhumtsev A, Ferguson A, Chang CT, Yusupov M, Blanchard SC, Yusupova G. Aminoglycoside interactions and impacts on the eukaryotic ribosome. PNAS (2017) Vol.114(51):E10899-E10908. PMID: 29208708.

Dyla M, Terry DS, Kjaergaard M, Andersen JL, Knudsen CR, Altman RB, Nissen P, Blanchard SC. Dynamics of P-type ATPase transport cycle revealed by single molecule FRET NATURE (2017). Vol.551(7860):346-351. PMID: 29144454.

Herschhorn A, Ma X, Gu C, Ventura JD, Castillo-Menendez L, Melillo B, Terry DS, Smith AB 3rd, Blanchard SC, Munro JB, Mothes W, Finzi A, Sodroski JG. Release of gp120 restraints leads to an entry-competent intermediate state of HIV-1 envelope glycoproteins. J. NATURE COMMUNICATIONS (2017) Vol.8(1).1049. PMID: 29051495.


Alejo JL, Blanchard SC. Miscoding-Induced stalling of substrate translocation on the bacterial ribosome. PNAS (2017). Vol.114(41):E8606-E8610. PMID:28973849.


Gregorio GG, Masureel M, Hilger D, Terry DS, Juette M, Zhao H, Zhou Z, Perez-Aguilar JM, Hauge M, Mathiasen S, Javitch JA, Weinstein H, Kobilka BK, Blanchard SC. Single-molecule analysis of ligand efficacy in β(2)AR-G-protein activation. NATURE.(2017) Vol.457(7661:68-73. PMID: 28607487.

Vušurović N, Altman RB, Terry DS, Micura R, Blanchard SC. Pseudoknot Formation Seeds the Twister Ribozyme Cleavage Reaction Coordinate.J AM CHEM SOC. (2017) Jun 9. doi: 10.1021/jacs.7b01549. [Epub ahead of print] PubMed PMID: 28598157.

Zheng Q, Jockusch S, Zhou Z, Altman RB, Zhao H, Asher W, Holsey M, Mathiasen S, Geggier P, Javitch JA and Blanchard SC Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging CHEMICAL SCIENCE (2017) Vol. 8(1):755-762




Herschhorn A, Ma X, Gu C, Ventura JD, Castillo-Menendez L, Melillo B, Terry DS, Smith AB 3rd, Blanchard SC, Munro JB, Mothes W, Finzi A, Sodroski JG. Release of gp120 Restraints Leads to an Entry-Competent Intermediate State of the HIV-1 Envelope Glycoproteins. MBIO. (2016) Vol.7 (5) pii:e01598-16. DOI: 10.1128/mBio.01598-16. PMID: 27795397

Engineering a prototypic P-type ATPase Listeria Monocytogenes Ca(2+)-ATPase 1 for single-molecule FRET studies. Dyla M, Andersen J, Kjaergaard M, Birkedal V, Terry DS, Altman RB, Blanchard SC, Nissen P, Knudsen CR. BIOCONJUGATE CHEMISTRY (2016) Aug 8. [Epub ahead of print] PubMed PMID: 27501274.

Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by  RNA Polymerase I. Dass RA, Sarshad AA, Carson BB, Feenstra JM, Kaur A, Obrdlik A, Parks MM, Prakash V, Love DK, Pietras K, Serra R, Blanchard SC, Percipalle P, Brown AM, Vincent CT. PLOS GENETICS 2016 Aug 8;12(8):e1006217. (2016) PubMed PMID: 27500936.

Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors. Cocozaki AI, Altman RB, Huang J, Buurman ET, Kazmirski SL, Doig P, Prince DB,  Blanchard SC, Cate JH, Ferguson AD. PNAS (2016) Jul 19;113(29):8188-93. (2016)  PMID: 27382179; PMCID: PMC4961145.

Structures of the orthosomycin antibiotics avilamycin and evernimicin in complex with the bacterial 70S ribosome. Arenz S, Juette MF, Graf M, Nguyen F, Huter P, Polikanov YS, Blanchard SC, Wilson DN. PNAS (2016) Jun 21. pii: 201604790. PMID: 27330110.

Effect of acidic pH on the stability of α-Synuclein dimers. Lv Z, Krasnoslobodtsev AV, Zhang Y, Ysselstein D, Rochet JC, Blanchard SC,
Lyubchenko YL. BIOPOLYMERS. (2016) May 14. PubMed PMID: 27177831.

Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody. Kong R, Xu K, Zhou T, Acharya P, Lemmin T, Liu K, Ozorowski G, Soto C, Taft JD, Bailer RT, Cale EM, Chen L, Choi CW, Chuang GY, Doria-Rose NA, Druz A, Georgiev IS, Gorman J, Huang J, Joyce MG, Louder MK, Ma X, McKee K, O'Dell S, Pancera M, Yang Y, Blanchard SC, Mothes W, Burton DR, Koff WC, Connors M, Ward AB, Kwong PD, Mascola JR.  SCIENCE. (2016) Vol.352(6287):828-33. PubMed PMID: 27174988.


Multi-perspective smFRET reveals rate-determining late intermediates of ribosomal translocation. Wasserman MR., Alejo JL, Altman RB, Blanchard SC. NATURE STRUCTURAL & MOLECULAR BIOLOGY (2016) Feb. 29th Edition.


Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale. Juette MF, Terry DS, Wasserman MR, Altman RB, Zhou Z, Zhao H, Blanchard SC. NATURE METHODS (2016) Vol. 13.


Genome sequence and analysis of Escherichia coli MRE600, a colicinogenic, nonmotile strain that lacks RNase I and the type I methyltransferase, EcoKI. Kurylo CM, Alexander N, Dass RA, Parks MM, Altman RB, Vincent CT, Mason CE, Blanchard SC. GENOME BIOLOGY AND EVOLUTION (2016) Vol. 8(3):742-752.


Intra-Molecular Triplet Energy Transfer is a General Approach to Improve Organic Fluorophore Photostability. Zheng Q., Jockusch S, Rodriguez-Calero GG, Zhou Z, Zhao H, Altman RB, Abruna HD, Blanchard SC. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES (2016) Vol. 15:196-203. 



Functional Dynamics within the Human Ribosome Regulate the Rate of Active Protein Synthesis. Ferguson A, Wang L, Altman RB, Terry DS, Juette MF, Burnett BJ, Alejo JL, DassRA, Parks MM, Vincent CT, Blanchard SC. MOLECULAR CELL (2015) Vol.60(3):475-86.


Chemically related 4,5-linked  aminoglycoside antibiotics drive subunit rotation in opposite directions. Wasserman MR, Pulk A, Zhou Z, Altman RB, Zinder JC, Green KD,Garneau-Tsodikova S, Doudna Cate JH, Blanchard SC. NATURE COMMUNICATIONS (2015) Vol.30;6:7896.


Assembly and diploid architecture of an individual human genome via single-molecule technologies. Pendleton M, Sebra R, Pang AW, Ummat A, Franzen O, Rausch T, Stütz AM, StedmanW, Anantharaman T, Hastie A, Dai H, Fritz MH, Cao H, Cohain A, Deikus G, Durrett RE, Blanchard SC, Altman R, Chin CS, Guo Y, Paxinos EE, Korbel JO, Darnell RB,McCombie WR, Kwok PY, Mason CE, Schadt EE, Bashir A. NATURE METHODS (2015) Vol.12(8):780-6


Crystal structure, conformational fixation and entry-related interactions ofmature ligand-free HIV-1 Env. Kwon YD, Pancera M, Acharya P, Georgiev IS, Crooks ET, Gorman J, Joyce MG, Guttman M, Ma X, Narpala S, Soto C, Terry DS, Yang Y, Zhou T, Ahlsen G, Bailer RT, Chambers M, Chuang GY, Doria-Rose NA, Druz A, Hallen MA, Harned A, Kirys T, Louder MK, O'Dell S, Ofek G, Osawa K, Prabhakaran M, Sastry M, Stewart-Jones GB,  Stuckey J, Thomas PV, Tittley T, Williams C, Zhang B, Zhao H, Zhou Z, Donald BR, Lee LK, Zolla-Pazner S, Baxa U, Schön A, Freire E, Shapiro L, Lee KK, Arthos J, Munro JB, Blanchard SC, Mothes W, Binley JM, McDermott AB, Mascola JR, Kwong PD. NATURE STRUCTURAL & MOLECULAR BIOLOGY (2015) Vol.22(7):522-31.


Distinct tRNA accommodation intermediates observed on the ribosome with the antibiotics Hygromycin A and A201A. Polikanov YS, Starosta AL, Juette MF, Altman RB, Terry DS, Lu W, Burnett BJ, Dinos G, Reynolds KA, Blanchard SC, Steitz TA, Wilson DN. MOLECULAR CELL (2015) Vol. 58:1-13.


Direct detection of alpha-Synuclein dimerization dynamics: Single-Molecule Fluorescence Analysis. Lv Z, Krasnoslobodtsev AV, Zhang Y, Ysselstein D, Rochet JC, Blanchard SC, Lyubchenko YL. BIOPHYSICAL JOURNAL (2015) Vol. 108(8):2038-2047.


High-resolution structure of the Escherichia coli ribosome. Noeske J, Wasserman MR, Terry DS, Altman RB, Blanchard SC, Cate JH. NATURE STRUCTURAL & MOLECULAR BIOLOGY (2015) Vol. 22(4):336-341.


Transport domain unlocking sets the uptake rate of an aspartate transporter. Akyuz N, Georgieva ER, Zhou Z, Stolzenberg S, Cuendet MA, Khelashvili G, Altman RB, Terry DS, Freed JH, Weinstein H, Boudker O, Blanchard SC. NATURE (2015) 518(7537):68-73.


Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome. Olivier NB, Altman RB, Noeske J, Basarab GS, Code E, Ferguson AD, Gao N, Huang J, Juette MF, Livchak S, Miller MD, Prince DB, Cate JH, Buurman ET, Blanchard SC. PNAS (2014) 111(46):16274-16279.


Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions. Munro JB, Gorman J, Ma X, Zhou Z, Arthos J, Burton DR, Koff WC, Courter JR, Smith AB 3rd, Kwong PD, Blanchard SC, Mothes W. SCIENCE (2014) 346(6210):759-763.


Structure and immune recognition of trimeric pre-fusion HIV-1 Env.Pancera M, Zhou T, Druz A, Georgiev IS, Soto C, Gorman J, Huang J, Acharya P, Chuang GY, Ofek G, Stewart-Jones GB, Stuckey J, Bailer RT, Joyce MG, Louder MK, Tumba N, Yang Y, Zhang B, Cohen MS, Haynes BF, Mascola JR, Morris L, Munro JB, Blanchard SC, Mothes W, Connors M, Kwong PD. NATURE (2014) 514(7523):455-461.


Direct evidence of an elongation factor-Tu/Ts·GTP·Aminoacyl-tRNA quaternary complex. Burnett BJ, Altman RB, Ferguson A, Wasserman MR, Zhou Z, Blanchard SC. J. BIOL. CHEM. (2014) 289(34):23917-23927.


The bright future of single-molecule fluorescence imaging. Juette MF, Terry DS, Wasserman MR, Zhou Z, Altman RB, Zheng Q, Blanchard SC. CURR. OPIN. CHEM. BIOL. (2014), 20:103-111.


The contribution of reactive oxygen species to the photobleaching of organic fluorophores. Zheng Q, Jockusch S, ZhouZ, Blanchard SC. PHOTOCHEM PHOTOBIOL (2014), 90(2):448-454.


Ultra-stable organic fluorophores for single-molecule research. Zheng Q, Juette MF, Jockusch S, Wasserman MR, Zhou Z, Altman RB, Blanchard SC, CHEM SOC REV. (2014), 43(4):1044-1056.


The central role of protein S12 in organizing the structure of the decoding site of the ribosome. Demirci H, Wang L, Murphy FV, Murphy E, Carr JF, Blanchard SC, Jogl G, Dahlberg AE, Gregory ST. RNA (2013) 19:1791-1801.


Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis. Jenner L, Starosta AL, Terry DS, Mikolajka A, Filonava L, Yusupov M, Blanchard SC, Wilson DN, Yusupova G, PNAS (2013) 110(10):3812-3816.


Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution. Haller A, Altman RB, Souliere MF, Blanchard SC, Micura R, PNAS (2013) 110(11):4188-4193.


Elongation factor Ts directly facilitates the formation and disassembly of the Escherichia coli elongation factor TuGTPaminoacyl-tRNA ternary complex. Burnett BJ, Altman RB, Ferrao R, Alejo JL, Kaur N, Kanji J, Blanchard SC, J BIOL CHEM (2013), 288(19):13917-13928.


Connecting the kinetics and energy landscape of tRNA translocation on the ribosome. Whitford PC, Blanchard SC, Cate JH, Sanbonmatsu KY. PLOS COMPUT BIOL (2013), 9(3):e1003003.


Small-molecule photostabilizing agents are modifiers of lipid bilayer properties. Alejo JL, Blanchard SC, Andersen OS. BIOPHYS J (2013) 104(11)2410:2418.


Transport dynamics in a glutamate transporter homologue. Akyuz N, Altman RB, Blanchard SC, Boudker O. NATURE (2013), 502(7469):114-118.


Tuning a riboswitch response through structural extension of a pseudoknot. Souliere MF, Altman RB Schwarz V, Haller A, Blanchard SC, Micura R. PNAS (2013) 110(35):E3256-3264.


The central role of protein S12 in organizing the structure of the decoding site of the ribosome. Demirci H, Wang L, Murphy FB 4th, Murphy EL, Carr JF, Blanchard SC, Jogl G, Dahlberg AE, Gregory ST. RNA (2013), 19(12):1791-1801.


Allosteric Control of the Ribosome by Small-molecule Antibiotics. Wang L., Pulk A., Wasserman MR., Feldman MB., Altman RB., Doudna Cate JH., Blanchard SC., NATURE STRUCTURAL & MOLECULAR BIOLOGY (2012): doi: 10.1038/nsmb.2360.


On the Mechanisms of Cyanine Fluorophore Photstablization. Zheng Q., Jockusch S., Zhou Z., Altman RB., Warren JD., Turro NJ., Blanchard SC., J. PHYS. CHEM. LETT. (2012): Vol.3:2200-2203.


Enhanced Photostability of Cyanine Fluorophores across the Visible Spectrum. Altman RB., Zheng Q., Zhou Z., Terry DS., Warren JD., Blanchard SC., NATURE METHODS (2012): Vol.9(1):68-71. PMID:22543373. 

Reply to "'Self-healing' Dyes: Intramolecular Stabilization of Organic Fluorophores". Blanchard SC., NATURE METHODS (2012): Vol.9(1):68-71. PMID:22543372.


Mechanistic insights into antibiotic action on the ribosome through single-molecule fluorescence imaging. Wang L, Wasserman MR, Feldman MB, Altman RB, Blanchard SC, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (2011) doi: 10.1111/j. 1749-6632.2012.x


Cyanine Fluorophore Derivatives with Enhanced Photostability. Altman RB, Terry DS, Zhou Z, Zheng Q, Geggier P, Kolster RA, Zhao Y, Javitch JA, Warren JD, Blanchard SC, NATURE METHODS (2011) 9(1):68-71.


Structure and Dynamics of the Mammalian Ribosomal Pretranslocation Complex. Budkevich T., Giesebrecht J., Altman RB., Munro JB., Mielke T., Nierhaus KH., Blanchard SC., Spahn CM., MOLECULAR CELL (2011) Vol. 44(2):214-224. PMID: 22017870.


Insights into the Molecular Determinants of EF-G Catalyzed Translocation. Wang L., Altman RB., Blanchard SC. RNA (2011) Vol.17(12:2189-2200. PMID: 22033333.


Dynamic Views of Ribosome Function: Energy Landscapes and Ensembles. Whitford PC., Altman RB., Geggier P., Terry DS., Munro JB., Onuchic JN., Spahn CMT., Sanbonmatsu KY., Blanchard SC. RIBOSOMES: STRUCTURE, FUNCTION AND DYNAMICS Rodnina M., Wintermeyer W., Green R (Eds)1st Ed., XII: ISBN 978-3-7091-0214-5 (2011).


Information Processing by Nanomachines. Sanbonmatsu KY., Blanchard SC., Whitford PC. MOLECULAR MACHINES.


Structures of the Bacterial Ribosome in Classical and Hybrid States of tRNA Binding. Dunkle, JA, Wang L, Feldman MB, Pulk A, Chen V, Kapral G, Noeske J, Richardson JS, Blanchard SC, Cate JHD. SCIENCE (2011) Vol. 332: 981-984. PMID: 21596992.


Conformational Capture of the SAM-II Riboswitch. Haller A, Rieder U, Aigner M, Blancahrd SC, Micura R. NATURE CHEM BIOL (2011) Vol.7(6):393-400. PMID: 21132598.


Substrate-Modulated Gating Dynamics in a Na(+)-coupled Neurotransmitter Transporter Homologue. Zhao Y, Terry DS, Shi L, Quick M, Weinstein H, Blanchard SC, Javitch JA. NATURE (2011) : Vol.474(7349):109-113. PMID: 21516104.


Cryoelectron Microscopy Structure of the Ribosome Complex in Intermediate States during tRNA Translocation. Fu J, Munro JB, Blanchard SC, Frank J. PNAS (2011) Vol. 108(12): 4817-21: PMID: 21383139.


Correlated Conformational Events in EF-G and the Ribosome Regulate Translocation. Munro JB, Wasserman MR, Altman RB, Wang L, Blanchard SC NATURE STRUCTURAL & MOLECULAR BIOLOGY (2010) Vol. 17(12):1470-1477. PMID: 21057527.


Probing Translation with Small-Molecule Inhibitors. Blanchard SC, Cooperman BS, Wilson DN; ACS CHEM BIOL (2010) Vol. 17(6): 633-645. PMID: 20609413.


Conformational Sampling of Aminoacyl-tRNA during Selection on the Bacterial Ribosome. Geggier PE, Dave R, Feldman MB, Terry D, Altman RB, Blanchard SC; J MOL BIOL (2010) Vol. 399:576-595. PMID:20434456.


Single-Molecule Dynamics of Gating in a Neurotransmitter Transporter Homologue. Zhao YZ, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA; NATURE (2010) Vol. 465(7295):188-93. PMID: 20463731.


Accommodation of Aminoacyl-tRNA into the Ribosome Involves Reversible Excursions along Multiple Pathways. Whitford PC, Geggier P, Altman RB, Blanchard SC, Onuchic JN, Sanbonmatsu KY; RNA (2010) Vol. 16(6): 1196-204. PMID: 20427512.


A Fast Dynamic Mode of the EF-G Bound Ribosome. Munro JB, Altman RB, Tung C-S, Sanbonmatsu KY, Blanchard SC; EMBO J (2010) Vol. 29(4): 770-81. PMID: 20033061.


Spontaneous Formation of the Unlocked State of the Ribosome is a Multistep Process. Munro JB., Altman RB, Tung CS, Cate JHD, Sanbonmatsu KY, and Blanchard SC; PNAS (2010) Vol. 102 (2): 709-714. PMID: 20018653.


Aminoglycoside Activity Observed on Single Pre-translocation Ribosome Complexes. Feldman MB, Terry DS, Altman RB, Blanchard SC; NATURE CHEM BIOL (2010) Vol. 6(1): 54-62. PMID: 19946275.


Navigating the Ribosome's Metastable Energy Landscape. Munro JB, Sanbonmatsu, KY, Spahn CMT, and Blanchard SC; TRENDS BIOCHEM. SCI (2009) Vol. 34(8):390-400. PMID: 19647434.


When Starting Over Makes More Sense. Blanchard SC; ACS CHEM BIOL (2009) Vol. 4(3): 89-92. PMID: 19228069. 

Single-Molecule Observations of Ribosome Function. Blanchard SC; CURR OPIN STRUC BIOL (2009) Vol. 19:1-7. PMID: 19223173.


Mitigating Unwanted Photophysical Processes for Improved Single-Molecule Fluorescence Imaging. Dave R, Terry DS, Munro JB, Blanchard SC; BIOPHYS J (2009) Vol. 96(6):2371-2381. PMID: 19289062.


Single-Molecule Studies of Biomolecules. Blanchard SC., Altman RB., Geggier P., Munro JB., Dave R., Feldman MB., Terry DS. WILEY ENCYCLOPEDIA OF CHEMICAL BIOLOGY (2008) DOI: 10.1002/9780470048672.wecb540.


Breaking the Barriers of Translation. Blanchard SC; NATURE CHEM BIOL (2008) pp 275-276. PMID: 18421289. 

A New View of Protein Synthesis: Mapping the Free Energy Landscape of the Ribosome using Single-Molecule FRET. Munro JB, Vaiana A, Sanbonmatsu KY, Blanchard SC. BIOPOLYMERS (2008) Vol. 89, No. 7 pp 565-577. PMID: 18286627.


Identification of Two Distinct Hybrid State Intermediates on the Ribosome. Munro JB. , Altman RB, O'Connor N, Blanchard SC. MOLECULAR CELL (2007) Vol.25. pp. 505-517. PMID: 17317624.


The Role of Fluctuations in tRNA Selection by the Ribosome. Lee TH, Blanchard SC, Kim HD, Puglisi JD, Chu S. PNAS (2007) Vol. 104, No. 34 pp 13661-13665. PMID: 17699629.


Site-specific Labeling of the Ribosome for Single-Molecule Spectroscopy. Dorywalska M, Blanchard SC, Gonzalez RL Jr., Kim HD, Chu S, Puglisi JD. NUCLEIC ACIDS RESEARCH (2005) Vol. 33 No. 1, pp. 182-189. PMID: 15647501. 


tRNA Selection and Kinetic Proofreading in Translation. Blanchard SC, Kim HD, Gonzalez RL Jr., Chu S Puglisi JD. NATURE STRUCTURAL AND MOLECULAR BIOLOGY (2004) Vol. 11, No. 10, pp. 1008-1014. PMID: 15448679. 


tRNA Dynamics on the Ribosome During Translation. Blanchard SC, Gonzalez RL Jr., Kim HD, Puglisi JD, Chu S. PNAS (2004) Vol. 101, No. 35, pp. 12893-12898. PMID: 15317937.


Solution Structure of the A loop of 23S ribosomal RNA. Blanchard SC and Puglisi JD. PNAS (2001) Vol. 98, No. 7, pp. 3720-3725. PMID: 11259644.


Approaching Translation at Atomic Resolution. Puglisi JD, Blanchard SC, Green RA. NATURE STRUCTURAL BIOLOGY (2000) Vol. 7, No. 10 pp.855-861. PMID: 11017192.


Aminoglycoside Antibiotics and Decoding. Joseph D. Puglisi, Scott C. Blanchard, Kam D. Dahlquist, Robert G. Eason, Dominique Fourmy, Steven R. Lynch, Michael I. Recht, and Satoko Yoshizawa, In The Ribosome: Structure, Function, Antibiotics, and Cellular Interactions, Edited by R.A. Garrett, S.R. Douthwaite, A. Liljas, A.T. Matheson, P.B. Moore, and H.F. Noller (2000) ASM Press, Washington, D.C.


rRNA Chemical Groups Required for Aminoglycoside Binding. Blanchard SC, Fourmy D, Eason RG, Puglisi JD. BIOCHEMISTRY (1999) Vol. 37, No. 21 pp. 7716-7724. PMID: 9601031.


Structure of the A Site of Esherichia coli 16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic. Fourmy D, Recht MI, Blanchard SC, Puglisi JD. SCIENCE (1996) Vol. 274, pp. 1367-1371. PMID: 8910275.


RNA Sequence Determinants for Aminoglycoside Binding to an A-site rRNA Model Oligonucleotide. Recht MI, Fourmy D, Blanchard SC, Dahlquist K, Puglisi JD. JOURNAL OF MOLECULAR BIOLOGY (1996) Vol. 262 pp. 421-436. PMID: 8893854.


Solution Structure of a Bovine Immunodeficiency Virus Tat-TAR Peptide-RNA Complex. Puglisi JD, Chen L, Blanchard SC, Frankel AD, SCIENCE (1994) Vol. 270, pp. 1200-1203. PMID: 7502045.

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